Assay for Neural Induction in the Chick Embryo
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چکیده
منابع مشابه
Assay for Neural Induction in the Chick Embryo
The chick embryo is a valuable tool in the study of early embryonic development. Its transparency, accessibility and ease of manipulation, make it an ideal tool for studying the formation and initial patterning of the nervous system. This video demonstrates how to graft organizer tissue into a host, a method by which Hensen s node (the organizer in the chick embryo) is grafted to a host compete...
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Induction and regionalisation of the chick nervous system were investigated by transplanting Hensen's node into the extra-embryonic region (area opaca margin) of a host embryo. Chick/quail chimaeras were used to determine the contributions of host and donor tissue to the supernumerary axis, and three molecular markers, Engrailed, neurofilaments (antibody 3A10) and XlHbox1/Hox3.3 were used to ai...
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The egg 50% lethal doses (IiD5o) for Coxiella burnetii range from 105 to 10-6, whereas the 50% infectious doses (ID5o) determined by microscopic examination range from 10-10 to 10-11 (Weiss and Pietryk, J. Bacteriol. 72:235, 1956; Pickens and Gaon, Am. J. Trop. Med. Hyg. 10:49, 1961). If the LDno value for eggs could be increased, dilute suspensions of rickettsiae could be assayed without the t...
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Previous studies on neural induction have identified regionally localized inducing activities, signaling molecules, potential competence factors and various other features of this important, early differentiation event. In this paper, we have developed an improved model system for analyzing neural induction and patterning using transverse blastoderm isolates obtained from gastrulating chick emb...
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To begin to reconcile models of floor plate formation in the vertebrate neural tube, we have performed experiments aimed at understanding the development of the early floor plate in the chick embryo. Using real-time analyses of cell behaviour, we provide evidence that the principal contributor to the early neural midline, the future anterior floor plate, exists as a separate population of floor...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2009
ISSN: 1940-087X
DOI: 10.3791/1027